Advancing Biomedical Science through Tissue Arrays |
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Dedicated facility |
Antibody Characterization
IHC
Profiling & Antibody Characterization - Patterns, Specificity and Normal
Vs Tumor
Orders and
Inquiries
Email:
service@pantomics.com
or
info@pantomics.com
Tel: 1-415-863-2380 or
1-510-207-9788
Fax:1-510-653-1227
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An Example of Antibody Characterization |
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Background:
Molecule A has
been known to express on the epithelial cell membrane of human
gastric mucosa. It has also been shown to express on the cell
membrane of certain types of carcinomas. Two types (mouse and
human) of monoclonal antibodies were generated against the human
type molecule A. To explore the therapeutic potential of the
human antibody and the target, it was important to determine: 1.
expression pattern of the molecule among a variety of normal and
tumor tissues; 2. cross-reactivity of the human monoclonal
antibody in human tissues. Biotinylated antibodies were
prepared. IHC was performed in tissue arrays containing 135
human normal tissues from
34 sites and 95 tumor tissues from 22 cancer types. |
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| Procedures | Results | Picture |
| IHC result analysis | Example of a chart showing detailed analysis of the antibody staining patterns across a wide range of normal and tumor tissues. |
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| IHC with mouse and human antibodies to molecule A in normal tissues | Example of the mouse (MMab, left panel) and human (HMab, right panel) antibody staining in normal tissues. Both antibodies gave strong membrane staining to the glandular epithelial cells in the stomach and ileum. Human antibody also showed cytoplasmic and non-specific staining to other cell types. |
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| IHC with mouse and human antibodies to molecule A in tumor tissues | In some types of tumor, the mouse antibody (left panel) showed specific , but heterogeneous membrane staining. The human antibody (right panel) showed membrane and cytoplasmic staining with non-specific background. |
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| IHC with mouse and human antibodies to molecule A in tumor tissues | In some other types of tumor, the mouse antibody (left panel) showed nuclear staining. The human antibody (right panel) showed predominantly membrane and cytoplasmic staining with non-specific background. |
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| Conclusion |
•The
mouse monoclonal antibody detected a general elevation
of molecule A
in tumors.
The staining was highly heterogeneous,
i.e. the patterns, positive rates and staining intensity
of tumor cells varied greatly among different and/or
same types of tumors, or within the same tumors.
•The human
antibody staining was predominantly cytoplasmic and
homogeneous. The antibody showed a low specificity to
its intended target cells on human tissue sections.
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Pantomics, Inc.
457 Mariposa Street, San Francisco, CA
94107, USA.
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