An Example of Pathology Model for Study of Asthma
Background: Goblet cell hyperplasia is a common pathological feature of Asthma. Using asthmatic tissues with typical goblet cell hyperplasia and IHC staining, we sought to identify molecules and inflammatory cells that might be related to this pathological feature of asthma.
A number of asthma cases with typical goblet cell hyperplasia feature were selected. Image on the left showing transitional changes from typical goblet cell hyperplasia to the relatively normal mucosal epithelial cells of the lung in a case of asthma
IHC staining for subtype X of T-cells
Subtype X T cells were mainly located under the bronchial epithelium. The number of the T cells correlated with the severity of the goblet cell hyperplasia.
IHC staining for subtype Y of T-cells
Subtype Y T cells were mainly located in the bronchial epithelium. The number of the T cells correlated with the severity of the goblet cell hyperplasia.
IHC detection of molecule B
IHC showed moderate to high expression of molecule B in the hyperplastic epithelial cells of the bronchia with no detectable level in the normal epithelial cells.
Our IHC study indicated that increase in number of type X and type Y (especially the later) and expression of molecule B might be associated the goblet cell hyperplasia in Asthma.