An Example of Pathology Model for Study of Asthma

Background: Goblet cell hyperplasia is a common pathological feature of Asthma. Using asthmatic tissues with typical goblet cell hyperplasia and IHC staining, we sought to identify molecules and inflammatory cells that might be related to this pathological feature of asthma.

 Procedures

 Results

Picture

 

 

 

Case selection

A number of asthma cases with typical goblet cell hyperplasia feature were selected. Image on the left showing transitional changes from typical goblet cell hyperplasia to the relatively normal mucosal epithelial cells of the lung in a case of asthma

 

 

 

IHC staining for subtype X of T-cells

 

 

Subtype X T cells were mainly located under the bronchial epithelium.  The number of the T cells correlated with the severity of the goblet cell hyperplasia.

 

 

 

IHC staining for subtype Y of T-cells

 

Subtype Y T cells were mainly located in the bronchial epithelium.  The number of the T cells correlated with the severity of the goblet cell hyperplasia.

 

 

 

IHC detection of molecule B

 

IHC showed moderate to high expression of molecule B in the hyperplastic epithelial cells of the bronchia with no detectable level in the normal epithelial cells.

 

 

 

Conclusion

Our IHC study indicated that increase in number of type X and type Y (especially the later) and expression of molecule B might be associated the goblet cell hyperplasia in Asthma.

PANTOMICS, INC.

1049 Union Ave. Suite D

Fairfield, CA 94533

Tel:  (415) 863-2380

Fax: (510) 225-1084

E-mail: sales@pantomics.com

               info@pantomics.com

© 2020 Pantomics Inc.