An Example of Tissue Cross-Reactivity IHC Study on  Normal Human Tissues

 Background: Studies in vitro study had showed that molecule Y was secreted in an abundant amount by tumor cells. The purpose of this profiling

 study was to determine the distribution of the molecule in various normal human tissues and evaluate suitability of the molecule as a target for

 antibody therapeutics. 

 Procedures

 Results

Picture

 

 

 

Antibody production

 

 

Specific antibody was produced. The antibody recognized a single band with correct molecular weight in Western blot (as indicated by the arrow).

 

 

 

IHC tissue profiling was performed in standard normal tissue arrays.

 

 

IHC tissue profiling showed a dominant cytoplasmic staining pattern of the molecule in a wide range of organs of the human body.

 

 

 

Example image of IHC profiling

 

 

 

IHC showed high expression level of molecule Y in the glandular cells of the pancreas.

 

 

 

Example image of IHC profiling

 

 

 

IHC showed high expression level of molecule Y in the glandular cells of the pituitary.

 

 

 

Example image of IHC profiling

 

 

 

IHC showed high expression level of molecule Y in the tubular epithelial cells of the kidney.

Conclusion

Our IHC tissue profiling showed a dominant cytoplasmic staining pattern of the molecule in a wide range of organs/tissues of the human body. The high expression levels of the molecule found  in many vital organs indicate that the molecule is not suitable as a target for antibody therapeutics.

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