IMAGING / PATHOLOGY ANALYSIS
Imaging and Whole Slide Scanning
We offer image acquisition and whole slide scanning services to medical and pharmaceutical customers and accept orders of only a few microscope slides to thousands of slides. Just send us your slides and you will get them back along with high quality digital images in a storage media.
1. Bright field and fluorescence image acquisition with top quality digital camera and microscope objectives. Images can be emailed, uploaded to cloud or a storage media;
2. Whole slide bright field scanning using Aperio ScanScope AT Turbo with a 20X objective (40X digital capability). Images can be uploaded to cloud or a storage media, which can then be viewed by Aperio’s free ImageScope Viewer.
Semi-quantitative IHC staining Analysis by Direct Microscope Observation
Direct scoring by eyes under a microscope is the most preferred and least expensive method for IHC, IF and ISH staining analysis in pathological, pre-clinical and clinical research. We provide the following semi-quantitative analyses for nuclear, membrane and cytoplasmic markers on cell smears/spins and tissue sections:
1. Basic analysis – including negative (- or 0), weak (+ or 1), moderate (++ or 2) and strong (+++ or 3) for average staining intensity, and positive rate (%) of the target cells across the section under analysis.
2. Semi-quantitative H-Score analysis - This is obtained by the formula: (3 x percentage of strongly stained target cells) + (2 x percentage of moderately stained target cells) + (1 x percentage of weakly stained target cells), giving a range of 0 to 300.
3. Detailed semi-quantitative analysis – Detailed staining intensity results are recorded as: “0” is negative. “0.5“ is borderline staining with no significance. “1” is weak staining. “1.5” is weak staining with foci of moderate staining. “2“ is moderate staining. “2.5“ is moderate staining with foci of strong staining. “3“ is homogeneous strong staining. “3.5“ is very strong and homogeneous staining with no significant background. “4” is over staining usually with background staining. Any figure in between can be described towards the description of the higher end, for example, 2.7 can be considered as strong staining. The average positive rate (%) of the target cells across each section is also recorded.
Quantitative Image Analysis by Computer Software
We have tested and set up for a number of image analysis software from leading companies including Aperio, Halo, Visionpharm and ImagePro. We can provide accurate quantitative analysis for nuclear, membrane and cytoplasmic markers identified by IHC, IF, ISH or special staining on cell smears/spins and tissue sections.
1. Cell number counting - positive cells per section, per TMA core or per high power view;
2. H-Score analysis – Software will identify and record negative (- or 0), weak (+ or 1), moderate (++ or 2) and strong (+++ or 3) staining intensity, and positive rate (%) of each intensity category for the target cells across the section or view under analysis. H-Score is then calculated by the formula: (3 x percentage of strongly stained target cells) + (2 x percentage of moderately stained target cells) + (1 x percentage of weakly stained target cells), giving a range of 0 to 300.
3. Positive IHC (protein), ISH (RNA) or special staining area vs negative area across the whole section or region of interest (ROI). Our software can identify different color, texture and contextual features, and segment them into appropriate areas (such as normal, tumor, necrosis, fibrosis or stromal areas) that can be analyzed (into percentage) and compared.