PanDoubleā¢ Immunofluorescence Mouse&Rabbit Ig Detection Kit
PanDouble Immunofluorescence Mouse&Rabbit Ig Detection Kit has the following features: 1. Designed to visualize mouse & rabbit primary antibodies simultaneously on human and goat frozen, paraffin tissue sections and cell smears. 2. Produces strong green (mouse Igs), red (rabbit Igs) or both fluorescence under a fluorescence microscope with a FITC, Taxes Red, or dual-band filter over a broad pH range (pH 4-9). 3. With our proprietary biotin-free signal amplification technology and auto-fluorescence quenching buffer formulation, the kit produces excellent signal-to-noise ratio.
Intended Use
For research use only, to visualize simultaneously mouse and rabbit Igs on human and goat frozen, paraffin tissue sections and cell smears.
Targets
Mouse&Rabbit Igs
Label
PanFluor 488, Green (mouse)+594, Red (rabbit)
Excitation / Emission
493/518 for green, 593/618 for red
Filter
FITC+Texas Red
References
"1) Hidotoshi Ino (2004) Application of antigen retrieval by heating for double-label fluorescent imunohistochemistry with identical species-derived primary antibodies. Journal of histochemistry & cytochemistry. Volume 52(9):1209-1217. 2) Chris M. van der Loos (2008) Multiple immunoenzyme staining: methods and visualization for observation with spectral imaging. Journal of histochemistry & cytochemistry. Volume 56(4):313-328. 3) Chris M. van der Loos etc (2000) The animal research kit (ARK) can be used in a multistep double staining method for human tissue specimens. Journal of histochemistry & cytochemistry. Volume 48(10): 1431-1437. 4) Viegas, MS et al. An improved and cost-effective methodology for the reduction of autofluorescence in direct immunofluorescence studies on formalinfixed paraffin-embedded tissues. European Journal of Histochemistry. 2007, Jan-Mar;51(1):59-66."
Downloads
Datasheet
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We also provide primary antibodies at $30 - $50 per slide