Alcian blue staining

Alcian blue stains acid mucosubstances and acetic mucins in paraffin-embedded tissue section. Excessive amounts of non-sulfated acidic mucosubstances are seen in mesotheliomas, certain amounts occur normally in blood vessel walls but increase in early lesions of atherosclerosis. Results: acid mucins/mucosubstances: blue; Nuclei (using nuclear fast red) redish pink.

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